Enum: LibraryGenerationMethod

Generic type of library generation.

URI: nfdi4immuno:LibraryGenerationMethod

Permissible Values

Value	Meaning	Description
pcr	None	Library generated using PCR amplification
rt_rhp_pcr	None	Reverse transcription using random hexamer primers followed by PCR
rt_oligo_d_t_pcr	None	Reverse transcription using oligo-dT primers followed by PCR
rt_oligo_d_t_ts_pcr	None	Reverse transcription using oligo-dT primers, template switching, followed by...
rt_oligo_d_t_ts_umi_pcr	None	Reverse transcription using oligo-dT primers, template switching with unique ...
rt_specific_pcr	None	Reverse transcription using locus-specific primers followed by PCR
rt_specific_ts_pcr	None	Reverse transcription using locus-specific primers, template switching, follo...
rt_specific_ts_umi_pcr	None	Reverse transcription using locus-specific primers, template switching with U...
rt_specific_umi_pcr	None	Reverse transcription using locus-specific primers with UMI, followed by PCR
rt_specific_umi_ts_pcr	None	Reverse transcription using locus-specific primers with UMI, template switchi...
rt_specific_ts	None	Reverse transcription using locus-specific primers followed by template switc...
other	None	Library generation method not covered by the above categories

Slots

Name	Description
library_generation_method	Generic type of library generation

Identifier and Mapping Information

Schema Source

• from schema: https://codebase.helmholtz.cloud/nfdi4immuno/portal-infra/metadata_schema

LinkML Source

name: LibraryGenerationMethod
description: Generic type of library generation.
title: Library generation method
from_schema: https://codebase.helmholtz.cloud/nfdi4immuno/portal-infra/metadata_schema
rank: 1000
permissible_values:
  pcr:
    text: pcr
    description: Library generated using PCR amplification.
    title: PCR
  rt_rhp_pcr:
    text: rt_rhp_pcr
    description: Reverse transcription using random hexamer primers followed by PCR.
    title: RT(RHP)+PCR
  rt_oligo_d_t_pcr:
    text: rt_oligo_d_t_pcr
    description: Reverse transcription using oligo-dT primers followed by PCR.
    title: RT(oligo-dT)+PCR
  rt_oligo_d_t_ts_pcr:
    text: rt_oligo_d_t_ts_pcr
    description: Reverse transcription using oligo-dT primers, template switching,
      followed by PCR.
    title: RT(oligo-dT)+TS+PCR
  rt_oligo_d_t_ts_umi_pcr:
    text: rt_oligo_d_t_ts_umi_pcr
    description: Reverse transcription using oligo-dT primers, template switching
      with unique molecular identifiers (UMI), followed by PCR.
    title: RT(oligo-dT)+TS(UMI)+PCR
  rt_specific_pcr:
    text: rt_specific_pcr
    description: Reverse transcription using locus-specific primers followed by PCR.
    title: RT(specific)+PCR
  rt_specific_ts_pcr:
    text: rt_specific_ts_pcr
    description: Reverse transcription using locus-specific primers, template switching,
      followed by PCR.
    title: RT(specific)+TS+PCR
  rt_specific_ts_umi_pcr:
    text: rt_specific_ts_umi_pcr
    description: Reverse transcription using locus-specific primers, template switching
      with UMI, followed by PCR.
    title: RT(specific)+TS(UMI)+PCR
  rt_specific_umi_pcr:
    text: rt_specific_umi_pcr
    description: Reverse transcription using locus-specific primers with UMI, followed
      by PCR.
    title: RT(specific+UMI)+PCR
  rt_specific_umi_ts_pcr:
    text: rt_specific_umi_ts_pcr
    description: Reverse transcription using locus-specific primers with UMI, template
      switching, followed by PCR.
    title: RT(specific+UMI)+TS+PCR
  rt_specific_ts:
    text: rt_specific_ts
    description: Reverse transcription using locus-specific primers followed by template
      switching.
    title: RT(specific)+TS
  other:
    text: other
    description: Library generation method not covered by the above categories.
    title: other