Enum: PhysicalLinkage
Mode of physical linkage between nucleic acids derived from distinct rearrangements before library preparation. All hetero_* terms indicate that in case of paired-read sequencing, the two reads should be expected to map to distinct IG/TR loci. *_head-head refers to techniques that link the 5' ends of transcripts in a single-cell context. *_tail-head refers to techniques that link the 3' end of one transcript to the 5' end of another one in a single-cell context. This term does not provide any information whether a continuous reading-frame between the two is generated. *_prelinked refers to constructs in which the linkage was already present on the DNA level (e.g. scFv).
URI: nfdi4immuno:PhysicalLinkage
Permissible Values
| Value | Meaning | Description |
|---|---|---|
| no_physical_linkage | None | No physical linkage between rearrangements |
| hetero_head_head | None | The 5' ends of transcripts are linked in a single-cell context |
| hetero_tail_head | None | The 3' end of one transcript is linked to the 5' end of another transcript in... |
| hetero_prelinked | None | The linkage was already present on the DNA level (e |
Slots
| Name | Description |
|---|---|
| physical_linkage | In case an experimental setup is used that physically links nucleic acids der... |
Identifier and Mapping Information
Schema Source
- from schema: https://codebase.helmholtz.cloud/nfdi4immuno/portal-infra/metadata_schema
LinkML Source
name: PhysicalLinkage
description: Mode of physical linkage between nucleic acids derived from distinct
rearrangements before library preparation. All `hetero_*` terms indicate that in
case of paired-read sequencing, the two reads should be expected to map to distinct
IG/TR loci. `*_head-head` refers to techniques that link the 5' ends of transcripts
in a single-cell context. `*_tail-head` refers to techniques that link the 3' end
of one transcript to the 5' end of another one in a single-cell context. This term
does not provide any information whether a continuous reading-frame between the
two is generated. `*_prelinked` refers to constructs in which the linkage was already
present on the DNA level (e.g. scFv).
title: Physical linkage of different rearrangements
from_schema: https://codebase.helmholtz.cloud/nfdi4immuno/portal-infra/metadata_schema
rank: 1000
permissible_values:
no_physical_linkage:
text: no_physical_linkage
description: No physical linkage between rearrangements.
title: No physical linkage
hetero_head_head:
text: hetero_head_head
description: The 5' ends of transcripts are linked in a single-cell context.
title: Hetero head-head
hetero_tail_head:
text: hetero_tail_head
description: The 3' end of one transcript is linked to the 5' end of another transcript
in a single-cell context.
title: Hetero tail-head
hetero_prelinked:
text: hetero_prelinked
description: The linkage was already present on the DNA level (e.g., scFv constructs).
title: Hetero prelinked